Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test - Université de Franche-Comté Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Microbiology Année : 2002

Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test

Akira Ito
  • Fonction : Auteur
Ning Xiao
  • Fonction : Auteur
Martine Liance
  • Fonction : Auteur
Marcello Sato
  • Fonction : Auteur
Yasuhito Sako
  • Fonction : Auteur
Wulamu Mamuti
  • Fonction : Auteur
Yuji Ishikawa
  • Fonction : Auteur
Minoru Nakao
  • Fonction : Auteur
Hiroshi Yamasaki
  • Fonction : Auteur
Kazuhiro Nakaya
  • Fonction : Auteur
Karine Bardonnet
  • Fonction : Auteur
Dominique Vuitton

Résumé

ABSTRACT Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out “blindly” using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available Echinococcus WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and Echinococcus WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em18 WB and Echinococcus WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys.

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Santé

Dates et versions

hal-03537147 , version 1 (20-01-2022)

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Citer

Akira Ito, Ning Xiao, Martine Liance, Marcello Sato, Yasuhito Sako, et al.. Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test. Journal of Clinical Microbiology, 2002, 40 (11), pp.4161-4165. ⟨10.1128/JCM.40.11.4161-4165.2002⟩. ⟨hal-03537147⟩

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