Practical Approach for Typing Strains of Leishmania infantum by Microsatellite Analysis - Archive ouverte HAL Access content directly
Journal Articles Journal of Clinical Microbiology Year : 2002

Practical Approach for Typing Strains of Leishmania infantum by Microsatellite Analysis

Béatrice Bulle
  • Function : Author
Jean-Mathieu Bart
  • Function : Author
Montserrat Gállego
  • Function : Author
Françoise Gambarelli
  • Function : Author
Montserrat Portús
  • Function : Author
Lee Schnur
  • Function : Author
Charles Jaffe
  • Function : Author
Salceda Fernandez-Barredo
  • Function : Author
José María Alunda
  • Function : Author
Béatrice Bulle
  • Function : Author
Montserrat Gállego
  • Function : Author
Françoise Gambarelli
  • Function : Author
Montserrat Portús
  • Function : Author
José María Alunda
  • Function : Author

Abstract

ABSTRACT Currently the universally accepted standard procedure for characterizing and identifying strains of Leishmania is isoenzyme analysis. However, in the Mediterranean area, despite their very wide geographical distribution, most Leishmania infantum strains belong to zymodeme MON-1. In order to increase our understanding of polymorphism in strains of L. infantum , we developed PCR assays amplifying 10 microsatellites and sequenced PCR products. The discriminative power of microsatellite analysis was tested by using a panel of 50 L. infantum strains collected from patients and dogs from Spain, France, and Israel, including 32 strains belonging to zymodeme MON-1, 8 strains belonging to zymodemes MON-24, MON-29, MON-33, MON-34, or MON-80, and 10 untyped strains. Five of the microsatellites were polymorphic, revealing 22 genotypes, whereas the five remaining microsatellites were not variable. In particular, MON-1 strains could be separated into 13 different closely related genotypes. MON-33 and MON-34 strains also gave two additional genotypes closely related to MON-1, while MON-29, MON-24, and MON 80 strains exhibited more divergent genotypes. Among the foci examined, the Catalonian focus displayed a high polymorphism, probably reflecting isoenzyme polymorphism, while the Israeli focus exhibited a low polymorphism that could be consistent with the recent reemergence and rapid spread of canine leishmaniasis in northern and central Israel. The strains originating from the south of France and the Madrid, Spain, area displayed significant microsatellite polymorphism even though they were monomorphic by isoenzyme analysis. In conclusion, microsatellite polymorphism exhibits a high discriminative power and appears to be suitable for characterization of closely related strains of L. infantum in epidemiological studies.

Dates and versions

hal-03534636 , version 1 (19-01-2022)

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Béatrice Bulle, Laurence Millon, Jean-Mathieu Bart, Montserrat Gállego, Françoise Gambarelli, et al.. Practical Approach for Typing Strains of Leishmania infantum by Microsatellite Analysis. Journal of Clinical Microbiology, 2002, 40 (9), pp.3391-3397. ⟨10.1128/JCM.40.9.3391-3397.2002⟩. ⟨hal-03534636⟩
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